What is Kinyoun stain used for?

Kinyoun, is a procedure used to stain acid-fast species of the bacterial genus Mycobacterium. It is a variation of a method developed by Robert Koch in 1882.

What type of stain is Kinyoun?

Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.

What is the primary stain in the Kinyoun acid-fast staining method?

The primary stain for the Kinyoun procedure is the aniline dye, basic fuchsin, that stains all the cells present red. The unique ability of mycobacteria to resist decolorization by acid-alcohol is why they are termed acid-fast, and will keep their red coloration throughout the staining process.

How can you tell the difference between Mycobacterium and Nocardia?

The laboratory diagnosis is based on microscopy and culture isolation, but Nocardia can be mistaken for Mycobacterium, as they not only grow in specific media for mycobacteria, but also form partially acid-fast beaded branching filaments, similar to those formed by rapidly growing mycobacteria18.

Why is Kinyoun considered cold method?

In 1915, Kinyoun published a method that has become known as the “cold staining” method because the heating step was removed in favor of using a higher concentration of the carbolfuchsin primary stain.

What is the wetting agent used in Kinyoun method?

The wetting agent, tergitol, reduces surface tension between the cell wall of the mycobacteria and the stain allowing it to penetrate. Why is heat or a surface-active agent used with the application of the primary stain during acid-fast staining?

What is the primary stain in the Kinyoun acid-fast stain quizlet?

What is the primary stain in acid fast stain? The primary stain in the Ziel-Neelsen method is carbol fuchsin, and basic fuchsin in the Kinyoun method.

How does Mycobacterium stain?

When looking at the smears for TB, it is stained using an acid-fast stain. These Acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids. These acids resist staining by ordinary methods such as a Gram stain.

Which stain is used for Mycobacterium?

Acid-Fast Bacteria—Ziehl– Neelsen Stain This stain is used to identify Mycobacterium tuberculosis, the causative agent of tuberculosis. Acid-fast organisms have a lipoid capsule that has a high molecular weight and is waxy at room temperature.

What is the primary stain in the Kinyoun acid-fast staining method How does this differ from the primary stain in the Ziehl-Neelsen method?

What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method? -the concentrations of the basic fuchsin (substituted for carbolfuchsin), and phenol are increased, making it unnecessary to heat the cells during the staining procedure.