What is a spectrum viewer?

The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover.

What wavelength is FITC?

FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.

How do you compensate FACS?

How To Compensate A 4-Color Flow Cytometry Experiment Correctly

1. 4 Steps To Compensating A 4-Color Experiment.
2. Choose the correct carrier for compensation.
3. Step 2: Collect the data and make sure there is a sufficient number of events.
4. Calculate compensation correctly.
5. Apply the compensation values and inspect the results.

What is Unmixing in flow cytometry?

Spectral Flow Cytometry. Home » Resources Library » Spectral Unmixing. Spectral unmixing is a mathematical algorithm that distinguishes the multiple fluorophore signatures within a multicolor tube and puts the each signature into its own parameter.

Is FITC a fluorescent dye?

Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility.

What is the difference between a fluorochrome and fluorophore?

As nouns the difference between fluorochrome and fluorophore is that fluorochrome is any of various fluorescent dyes used to stain biological material before microscopic examination while fluorophore is (biochemistry) a molecule or functional group which is capable of fluorescence.

What happens when UV light excites a fluorochrome?

During fluorescence imaging, the item being inspected absorbs a shorter wavelength of light (usually ultraviolet (UV) light) that excites a specific fluorophore, causing it to release photons that fluoresce and emit light at a longer wavelength. In industrial applications this fluorescence is most often a blue color.