What type of cell is HepG2?

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Where do HepG2 cells come from?

HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma, which is the fifth most-common cancer worldwide.

What are cytoplasmic vacuoles?

Cytoplasmic Vacuolation. Vacuoles are areas of the cytoplasm which do not stain with Wright’s stain and appear as holes in the cytoplasm. Their composition may vary; some will contain remnants of bacterial digestion, autodigestion in an aging cell, while others may contain fat.

What is vacuolation in biology?

A vacuole is a membrane-bound cell organelle. In animal cells, vacuoles are generally small and help sequester waste products. In plant cells, vacuoles help maintain water balance. Sometimes a single vacuole can take up most of the interior space of the plant cell.

Why are HepG2 cells used?

Hepatic cells are important in the drug development process since the liver is the main detoxifying organ in the human body and mainly responsible for drug metabolism and drug-drug interactions. Hence, HepG2 cells are widely used for drug development and toxicity testing.

What media is used for HepG2?

Medium: The Hep G2 [HEPG2] (HB-8065) cells require the Eagle’s Minimum Essential Medium (EMEM) (ATCC 30-2003) with 10% Fetal Bovine Serum (FBS) (ATCC 30-2020) that has not been heat inactivated.

Why is HepG2 used?

What causes cytoplasmic vacuolation?

Cytoplasmic vacuolization (also called cytoplasmic vacuolation) is a well-known morphological phenomenon observed in mammalian cells after exposure to bacterial or viral pathogens as well as to various natural and artificial low-molecular-weight compounds.

How do you pass HepG2 passage?

Culture HepG2 in EMEM=10%FCS to 80% confluence on T75. Trypsinize (5ml) , add equal volume (5ml complete media) into falcon tube and spin 1000g for 5 minutes. Aspirate supernatant and re-suspend in 1ml of complete media.